DIY Tissue Culture: How to Get Started for Less Than $200

I sacrificed a finger to bring you this video. please subscribe

Tissue Culture Starter Kit: https://plantsinjars.shop/products/starter-kit?srsltid=AfmBOooJYhIcwgvYaT6n22ZpEOlCbsau4-puMyTbXfWPHjM9VG6vSUH3
Beginner Lab Set up: https://amzn.to/3FLI3Tf
2025 Updated Beginner Tissue Culture Tutorial: https://www.youtube.com/watch?v=LTA8EHBNKPM

Join the Plants in Jars Community (AKA my free discord server full of plant nerds like you): https://discord.gg/p6yp2Ckv5g

Protocol for video: https://www.researchgate.net/publication/257148024_Micropropagation_of_self-heading_Philodendron_via_direct_shoot_regeneration

(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)

Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture